Reporter

Part:BBa_K2023016

Designed by: Célia Chenebault   Group: iGEM16_Ionis_Paris   (2016-10-14)


GFP coding device with Pu

This part allowed us to start the CelloCad side project.
In order to compare the RPU (Relative Promoter Unit) of the Pu promoter with the reference promoter BBa_J23101 we designed this part which is composed of Pu promoter (BBa_I723020), the RBS Elowitz 1999 (BBa_B0034), the GFP (BBa_E0040) and a double terminator (BBa_B0015).
To learn more about the CelloCad project, visit our website: http://2016.igem.org/Team:Ionis_Paris/Measurement


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 990


[edit]
Categories
//cds/reporter/gfp
//function/reporter/fluorescence
Parameters
emission511
excitation501
proteinGFP