Measurement
Part:BBa_K2023015
Designed by: Célia Chenebault Group: iGEM16_Ionis_Paris (2016-10-14)
XylR coding device-GFP coding device with Pu
This part allowed us to start the CelloCad side project.
In order to compare the RPU (Relative Promoter Unit) of the Pu promoter with the reference promoter BBa_J23101 we designed this part which is composed of the XylR coding device (BBa_K2023002) and the GFP coding device with Pu (BBa_K2023016).
To learn more about the CelloCad project, visit our website: http://2016.igem.org/Team:Ionis_Paris/Measurement
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2446
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2101
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3241
[edit]
Categories
Parameters
//cds/transcriptionalregulator/activator
//function/reporter/fluorescence
//function/reporter/fluorescence
control | Positive control with BBa_K2023007 |
emission | 511 |
excitation | 501 |
protein | XylR, GFP |