Measurement

Part:BBa_K2023015

Designed by: Célia Chenebault   Group: iGEM16_Ionis_Paris   (2016-10-14)


XylR coding device-GFP coding device with Pu

This part allowed us to start the CelloCad side project.
In order to compare the RPU (Relative Promoter Unit) of the Pu promoter with the reference promoter BBa_J23101 we designed this part which is composed of the XylR coding device (BBa_K2023002) and the GFP coding device with Pu (BBa_K2023016).
To learn more about the CelloCad project, visit our website: http://2016.igem.org/Team:Ionis_Paris/Measurement


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3241


[edit]
Categories
//cds/transcriptionalregulator/activator
//function/reporter/fluorescence
Parameters
controlPositive control with BBa_K2023007
emission511
excitation501
proteinXylR, GFP