Part:BBa_K2023013

XylR coding device with mRFP

This biobrick is composed with Pr (BBa_I723018), Elowitz 1999 RBS (BBa_B0034), XylR (BBa_K2023005), a second Elowitz 1999 RBS, mRFP (BBa_K1491009) and a double terminator (BBa_B0015). This part has been designed to prove that XylR protein is synthesized in the cells when the colonies becomes red thanks to the mRFP protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2101
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 329
  Illegal AgeI site found at 2721
  Illegal AgeI site found at 2833
  
• 1000
  COMPATIBLE WITH RFC[1000]