Composite

Part:BBa_K2023012

Designed by: Célia Chenebault   Group: iGEM16_Ionis_Paris   (2016-10-14)


Pr-RBS-XylR-His tag

This biobrick is composed with Pr promoter (BBa_I723018), the Elowitz RBS (BBa_B0034) and XylR gene with His tag (BBa_K2023010). XylR is optimized for E.coli DH5-alpha.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2101
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator/activator
Parameters
proteinXylR
tagHis