Device

Part:BBa_K2023007

Designed by: Célia Chenebault   Group: iGEM16_Ionis_Paris   (2016-10-14)


Gluc coding device with Pr

This GLuc coding device is made with the promoter Pr (BBa_I723018), the RBS Elowitz 1999 (BBa_B0034), the GLuc gene optimized for E.coli DH5 alpha (BBa_K2023009) and a double terminator (BBa_B0014). This part has been designed as a as the positive control in Gaussia Luciferase assay in the frame of a pollution quantification test with the biosensor device for BTEX detection (BBa_K2023001).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 530
    Illegal NheI site found at 941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter/light
Parameters
proteinGaussia Luciferase