Reporter

Part:BBa_K2023003

Designed by: Célia Chenebault   Group: iGEM16_Ionis_Paris   (2016-10-14)


Gluc coding device with Pu

This GLuc coding device is made with the promoter Pu (BBa_I723020), the RBS Elowitz 1999 (BBa_B0034), the GLuc gene optimized for E.coli DH5 alpha (BBa_K2023009) and a double terminator (BBa_B0014).
This part has been designed as a reporter for a biosensor device for BTEX detection (BBa_K2023001).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
    Illegal NheI site found at 440
    Illegal NheI site found at 851
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//cds/reporter
//function/reporter/light
Parameters
positive_regulators
proteinGaussia Luciferase