Regulatory
M5 TNN
Part:BBa_K202002
Designed by: Poonam Srivastava Group: iGEM09_Minnesota (2009-10-16)
Hybrid promoter having multiple operator sites.
Promoter was designed according to the Lutz R and Bujard H.(Nucleic Acids Res. 1997 Mar 15;25(6):1203-10). The operator sequences are from three unrelated natural regulatory elements: the tetracycline (tet), lactose (lac) and λ-phage operons arranged logically within a single transcriptional unit. The regulatory architecture is designed such that each operator's position efficiently interferes with RNA polymerase (RNAp) promoter binding without inhibiting promoter function. This promoter has tetO2 sites which are having transverse mutation at position 5. The hybrid promoter is cloned upstream to the GFP (part Bba_E0240) in plasmid pSB1A3.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 192
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |