Regulatory
M5 TNN

Part:BBa_K202002

Designed by: Poonam Srivastava   Group: iGEM09_Minnesota   (2009-10-16)

Hybrid promoter having multiple operator sites.

Promoter was designed according to the Lutz R and Bujard H.(Nucleic Acids Res. 1997 Mar 15;25(6):1203-10). The operator sequences are from three unrelated natural regulatory elements: the tetracycline (tet), lactose (lac) and λ-phage operons arranged logically within a single transcriptional unit. The regulatory architecture is designed such that each operator's position efficiently interferes with RNA polymerase (RNAp) promoter binding without inhibiting promoter function. This promoter has tetO2 sites which are having transverse mutation at position 5. The hybrid promoter is cloned upstream to the GFP (part Bba_E0240) in plasmid pSB1A3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 192
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None