Other
subE

Part:BBa_K2020000

Designed by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert   Group: iGEM16_Aachen   (2016-10-04)


subtilisin E gene

This DNA sequence can be used to express subtilisin E in Escherichia coli. 1

Usage and Biology

Subtilisin E is an alkaline serine protease which non-specifically digests proteins. It is naturally producedd by Bacillus subtilis.
To use this part a suitable leader sequence has to be placed in front as the sequence of this BioBrick does not contain a start codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/protease
//chassis/prokaryote/ecoli
Parameters
biologyBacillus subtilis
chassisEscherichia coli
proteinsubtilisin E