Coding

Part:BBa_K2019005

Designed by: John Lazar   Group: iGEM16_Northwestern   (2016-10-12)


Periplasm Directed saCas9 via Ycdo Signaling Sequence (Ycdo-saCas9)

Ycdo-Cas9 is a fusion of the Ycdo signaling sequence to the N-terminus of staphylococcus aureus CRISPR associated protein 9 (saCas9). Ycdo utilizes the sec pathway to direct the fused protein to the periplasm of the cell. Therefore, by fusing Cas9 to the C-terminus of the signaling sequence, it should be directed to the periplasm of the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 726
    Illegal BglII site found at 879
    Illegal BglII site found at 1025
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 299
    Illegal AgeI site found at 2855
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None