Composite

Part:BBa_K2015011

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


LacI expression unit

The part is a lacI expression unit. Because it contains a medium constitutive promoter (BBa_J23101) and RBS(BBa_B0032), lacI will be expressed moderately. pSB1C3 does not contain a lacI expression unit. If you insert the part into pSB1C3, you can regulate plac promoter strictly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None