Coding
Part:BBa_K2015009
Designed by: Kazuhiro Mimata, Ruka Nishimura Group: iGEM16_HokkaidoU_Japan (2016-10-14)
P11-4 for multimer
The part consists of Self-Assembling Region(SAR) and mutated GFP. SAR contains Short linker(SL, BBa_K2015004and P11-4(SAP,BBa_K2015001). It can be self-assembling by amino acids interaction and be covalently bonded between cysteine residues. It can form multimerized enzyme.
It contains mutated GFP which is deleted termination codon(TAATAA) from E0040. You can replace the part from GFP to other enzymes using BamHI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 67
Illegal BamHI site found at 787 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 716
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Categories
Parameters
None |