Coding

Part:BBa_K2015009

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


P11-4 for multimer


The part consists of Self-Assembling Region(SAR) and mutated GFP. SAR contains Short linker(SL, BBa_K2015004and P11-4(SAP,BBa_K2015001). It can be self-assembling by amino acids interaction and be covalently bonded between cysteine residues. It can form multimerized enzyme.
It contains mutated GFP which is deleted termination codon(TAATAA) from E0040. You can replace the part from GFP to other enzymes using BamHI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
    Illegal BamHI site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


[edit]
Categories
Parameters
None