Coding

Part:BBa_K2015008

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


RADA for multimer

The part consists of Self-Assembling Regions(SARs) and mutated GFP. SARs contain Short linker(SL, BBa_K2015004) and RADA16-I(BBa_K2015002). It has ability of self-assembling by amino acids interaction and it can be covalently bonded between cysteine regidues. Mutated GFP is deleted termination codon from BBa_E0040.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 82
    Illegal BamHI site found at 802
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


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Categories
Parameters
None