Composite
SAR

Part:BBa_K2015000

Designed by: Kazuhiro Mimata, Ruka Nishimura   Group: iGEM16_HokkaidoU_Japan   (2016-10-14)


Self-assembling region (SAR) for the circularization of proteins

This part contains Self-Assembling Regions(SARs) and it is a part of circularized enzyme. SARs have a potential to self-assemble by hydrophilic and hydrophobic amino acids under physiochemical conditions. It can express the circularized enzyme because it can insert the Zip-Up linker and an enzyme by cutting the multi-cloning sites.

This part contains plac(BBa_R0011), so it is induced by IPTG. And if you connect BBa_K201511 and this part, you can regulate more tightly because it contains a lacI unit. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 199
    Illegal BamHI site found at 211
    Illegal BamHI site found at 319
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None