Generator

Part:BBa_K2012024

Designed by: Boyao Zhang   Group: iGEM16_HZAU-China   (2016-10-07)


PcpcG2-B0034-PleD-His(6)Tag

Promoter cpcG2 is a 238bp green-light activated promoter from the genome of Synechocystis PCC6803(Part BBa_K592003).We acquire the sequence from Jeffrey Tabor in pJT119 plasmid and replace the reporter gene with PleD(Part:BBa_K2012002), a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
    Illegal BamHI site found at 1416
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 674
    Illegal NgoMIV site found at 821
    Illegal AgeI site found at 1157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 331


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Categories
Parameters
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