Composite

Part:BBa_K2010006

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-21)


PETase Composite (T7, His-Tag)

This composite part includes PETase regulated by a T7 promoter, a strong constitutive promoter, and also a his-tag for purification. Because of the T7 promoter, this part should be used in expression cells that are compatible with this promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 809
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1227


[edit]
Categories
Parameters
None