Part:BBa_K2008002

pVeg->sec-TD1-BBI-GFP

This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI). A B. subtilis secretory signal peptide sequence is included to allow for secretion of the peptide directly into surrounding media. A transdermal tag, TD1 (https://parts.igem.org/Part:BBa_K1074000) is fused to the N-terminus of BBI to allow for diffusion of the peptide across the skin.

This construct is fused to GFP (https://parts.igem.org/Part:BBa_E0040) for visual detection, and the entire coding sequence is under the control of the constitutive B. subtilis promoter pVeg (https://parts.igem.org/Part:BBa_K143012) and a strong B. subtilis RBS (https://parts.igem.org/Part:BBa_K780001).

Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 910