Part:BBa_K2008001

BBI with N-terminal KSCI solubility tag fused to GFP

This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. This component of the composite part is fused to the N-terminal end of GFP (https://parts.igem.org/Part:BBa_E0040) for visual detection, and is under the control of a constitutive E. coli promoter (https://parts.igem.org/Part:BBa_J23100) and strong RBS (https://parts.igem.org/Part:BBa_B0034).


Photograph of E. coli TOP10 colonies on petri dishes transformed with pSB1c3-K2008001 (left) and pSB1c3 (right). Colonies transformed with K2008001 (BBI-GFP) fluoresce green under UV light.

Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 742