Coding

Part:BBa_K2005090

Designed by: Jarrod Shilts   Group: iGEM16_Vanderbilt   (2016-10-18)


CAN1, UV-resistant

Coding sequence for CAN1 gene from yeast (Saccharomyces cerevisiae) with its sequence modified by our algorithm to minimize its susceptibility to mutation. This sequence has sufficient flanking homology regions to allow for integration into yeast, and features base modifications to accommodate CRISPR homology-directed repair. CAN1 codes for an arginine permease that also confers susceptibility to the drug canavanine.

We designed K2005090 as part of an in vivo selection system, where mutant cells could be positively enriched using canavanine to measure rates of mutation with our optimized genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 714
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 763
    Illegal NgoMIV site found at 1384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1327
    Illegal SapI.rc site found at 141


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Categories
Parameters
None