Composite

Part:BBa_K2004013

Designed by: Marco V. Lincango Y.   Group: iGEM16_BIOSINT_Mexico   (2016-10-18)


spisPink poly his tag

In this way, a brief step to confirm the chromoprotein expression is purifying it, so to facilitate this process we fuse a improved version of the sequence (BBa_K2004004) that codifies to pink chromoprotein with another registered part, the poly his tag (BBa_K844000). The last version of this chromoprotein(BBa_K1684000), designed by the previous Biosint team, was analyzed according to its sequence and all the parameter that must meet to be part of a standardized biobrick. This device (BBa_K2004013) allows to test the fusion, because contains a constitutive promoter and a RBS upstream and the double terminator downstream.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None