Composite

Part:BBa_K200027

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)

PAH+RBS+GFP+TT

Testing construct to assay the production of the PAH enzyme. The PAH enzyme is responsible for the breakdown of phenylalanine into tyrosine. This enzyme is produced by the Human liver and a genetic deficiency of the encoding gene can lead to a condition called Phenylketonuria. This condition causes mental retardation through progessive brain damage caused by the accumulation of Phenylalanine. More detailed information on the enzyme at the main PAH registry page: BBa_K200008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 814
    Illegal XhoI site found at 524
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2032


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Categories
Parameters
None