Part:BBa_K200001
Dam methylase -> Dam
The methylase encoded by the dam gene ([http://en.wikipedia.org/wiki/Dam_(methylase) Dam methylase]) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.
Usage and Biology
DAM methylase (DNA adenine methylase) adds a methyl group to the adenine of the sequence 5'-GATC-3'in newly synthesized DNA. Dam methylases are important for several biological functions:
- [http://en.wikipedia.org/wiki/DNA_replication DNA replication]
- [http://en.wikipedia.org/wiki/Mismatch_repair DNA mismatch repair]
- Regulation of protein expression.
In the E.ncapsulator project, Dam methylases are used to protect the DNA from being destroyed by restriction enzymes DpnII and TaqI.
The gene was used alongside with the restriction enzymes TaqI and the DpnII by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project as part of the genome deletion module. The Dam (methylase) is used to prevent genome restriction due to basal expression of the restriction enzymes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 306
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>#Dam1 http://fangman-brewer.genetics.washington.edu/hemimethylation.html</biblio>
n/a | Dam methylase -> Dam |