Coding

Part:BBa_K1999001

Designed by: Jimin Sun   Group: iGEM16_KoreaSonyeodul   (2016-10-10)


Mono(2-hydroxyethyl) terephthalate hydrolase (MHETase)

Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Catalyzes the hydrolysis of mono(2-hydroxyethyl) terephthalate into its two environmentally benign monomers, terephthalate and ethylene glycol. Does not show activity against PET, bis(hydroxyethyl) terephthalate (BHET), pNP-aliphatic esters or typical aromatic ester compounds catalyzed by the tannase family enzymes, such as ethyl gallate and ethyl ferulate.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1584
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1584
    Illegal NotI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
    Illegal BamHI site found at 424
    Illegal BamHI site found at 552
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1584
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1584
    Illegal NgoMIV site found at 55
    Illegal NgoMIV site found at 351
    Illegal NgoMIV site found at 1138
    Illegal NgoMIV site found at 1543
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 279


[edit]
Categories
Parameters
None