Coding
Part:BBa_K1999001
Designed by: Jimin Sun Group: iGEM16_KoreaSonyeodul (2016-10-10)
Mono(2-hydroxyethyl) terephthalate hydrolase (MHETase)
Involved in the degradation and assimilation of the plastic poly(ethylene terephthalate) (PET), which allows I.sakaiensis to use PET as its major energy and carbon source for growth. Catalyzes the hydrolysis of mono(2-hydroxyethyl) terephthalate into its two environmentally benign monomers, terephthalate and ethylene glycol. Does not show activity against PET, bis(hydroxyethyl) terephthalate (BHET), pNP-aliphatic esters or typical aromatic ester compounds catalyzed by the tannase family enzymes, such as ethyl gallate and ethyl ferulate.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1584
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1584
Illegal NotI site found at 253 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
Illegal BamHI site found at 424
Illegal BamHI site found at 552 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1584
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1584
Illegal NgoMIV site found at 55
Illegal NgoMIV site found at 351
Illegal NgoMIV site found at 1138
Illegal NgoMIV site found at 1543 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 279
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Categories
Parameters
None |