Composite

Part:BBa_K1994060

Designed by: Egheosa Ogbomo   Group: iGEM16_Warwick   (2016-10-14)


Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette

dCas9 with Pam staggered sequence and sigma 54 promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
    Illegal NheI site found at 2034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4313
    Illegal XhoI site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None