Composite

Part:BBa_K1994035

Designed by: Egheosa Ogbomo   Group: iGEM16_Warwick   (2016-10-14)


Multiple dCas9 binding site sequence with binding sequence positions shown

CRISPR sequence with recurring PAMs

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 225
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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