Composite

Part:BBa_K1994022

Designed by: Isobel Holden   Group: iGEM16_Warwick   (2016-10-14)


Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette

dCas9 with Pam staggered sequence and omega promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 225
    Illegal NheI site found at 331
    Illegal NheI site found at 2353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
    Illegal BamHI site found at 4632
    Illegal XhoI site found at 361
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 229
    Illegal AgeI site found at 397
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None