Device

Part:BBa_K199202

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-12-12)


pLac-RBS-ATG-loxP-STOP-loxP-TetA

Without Cre-mediated excision, TetA is not expressed. If the STOP sequence is excised, TetA is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 675
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 1215
    Illegal NgoMIV site found at 1375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 277


[edit]
Categories
Parameters
None