Device
Part:BBa_K199202
Designed by: Mary Gearing Group: iGEM09_MoWestern_Davidson (2010-12-12)
pLac-RBS-ATG-loxP-STOP-loxP-TetA
Without Cre-mediated excision, TetA is not expressed. If the STOP sequence is excised, TetA is expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 675
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 821
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 847
Illegal NgoMIV site found at 1215
Illegal NgoMIV site found at 1375 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 277
[edit]
Categories
Parameters
None |