Coding

Part:BBa_K199201

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-12-12)


pLac-RBS-ATG-loxP-STOP-loxP-RFP

Without Cre-mediated excision, RFP is not expressed. If the STOP sequence is excised, RFP is expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1086
    Illegal AgeI site found at 1198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 277


[edit]
Categories
Parameters
None