Composite

Part:BBa_K1992009

Designed by: Shilo Ohayon, Sharbel Zahran   Group: iGEM16_Technion_Israel   (2016-10-14)


Tar GFP tagged expression system (promoter+RBS+coding+terminator)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1343
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2385
    Illegal SapI.rc site found at 172

Introduction

A GFP gene was fused to the improved expression system of the Tar chemoreceptor(BBa_K1992004). The plasmid is comprised of a promoter, a strong RBS, Tar gene, a GFP gene and a terminator. In order to obtain the fusion, a proper linker sequence was introduced between the Tar and the GFP gene.

Usage and Biology

This device was used as a proof of concept in order to verify the migration of the chemoreceptor to the poles of the bacterial membrane.

Experiments and results

Expariments and results of this exprassion system can be seen in the Tar-GFP part (BBa_K1992003) and in our result page [http://2016.igem.org/Team:Technion_Israel/Tar_improvements Tar improvements and characterzation]


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