Part:BBa_K1992006

Histamin-Tar GFP tagged expression system (promoter+RBS+coding+terminator)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1355
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2411
  Illegal SapI.rc site found at 184
  

Introduction

A GFP gene was fused to the novel Histamine-Tar chemoreceptor (BBa_K1992011). The plasmid is comprised of a promoter, a native RBS (BBa_K1992000), the novel Histamine-Tar chemoreceptor (BBa_K1992011), a GFP gene and a terminator. In addition, a proper linker ought to be introduced in order to ensure the right correct fold of the protein.

Usage and Biology

This expression device was used as a proof of concept in order to verify the migration of the novel chemoreceptor to the poles of the bacterial membrane.

Experiments and results

For methods and results please go to the novel Histamine-Tar chemoreceptor (BBa_K1992011) or to our [http://2016.igem.org/Team:Technion_Israel/Proof proof of concept] page.