Reporter

Part:BBa_K199194

Designed by: Todd Eckdahl   Group: iGEM09_MoWestern_Davidson   (2010-09-06)


TT-pLux-RBS-LuxI 2 sites-RBS-GFP-RBS-RFP-pLacI-RBS-LuxR

pLacI drives LuxR gene, producing LuxR protein for activation of pLux promoter. pLux drives LuxI gene, producing enzyme to make 3OC6, required for LuxR activation. The circuit has amplification by the LuxI enzyme and positive feedback. pLux also drives GFP gene, followed by RFP gene. The LuxI gene is modified with restriction sites for AscI and AsiSI, for introduction of frameshift mutations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 483
    Illegal AgeI site found at 2175
    Illegal AgeI site found at 2287
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 135
    Illegal BsaI.rc site found at 1518


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Categories
Parameters
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