Device

Part:BBa_K199151

Designed by: Romina Clemente   Group: iGEM09_MoWestern_Davidson   (2010-05-05)


pLac-RBS-CUAGU-RFP-pLpp-CUAGU-tRNA

Reporter construct ligated upstream of a suppressor tRNA. The tRNA suppresses the 5 bp insert in the RFP gene. The promoter controlling tRNA production is pLpp, one of the strongest promoters in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1003
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 786
    Illegal AgeI site found at 898
    Illegal AgeI site found at 1040
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None