Coding

Part:BBa_K199125

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2010-03-16)

LuxI autoinducer synthetase optimized for E. coli

Synthesizes 3OC6HSL, which binds to LuxR. No LVA tag.

Directly downstream of the ATG is 21 base pairs translated into 7 amino acids not seen at the beginning of wild-type LuxI (C0161). This "optimized wild-type" sequence will be mutated to add an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites will allow for unique coding sequences to easily be swapped out of the gene. This "optimized wild-type" sequence acts as a positive control before adding in the restriction sites or additional coding sequences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 244
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aLuxI autoinducer synthetase optimized for E. coli