Coding

Part:BBa_K199123

Designed by: Romina Clemente   Group: iGEM09_MoWestern_Davidson   (2010-03-08)


autoinducer synthetase for AHL (no LVA) + Unique Site

Synthesizes 3OC6HSL, which binds to LuxR.

Directly downstream of the ATG start codon is an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites allow for unique coding sequences to easily be swapped out of the gene. These unique coding sequences contain frameshift mutations that can be suppressed by 5-bp tRNA suppressors. Whether or not these frameshift mutations are suppressed determines whether or not LuxI will be transcribed properly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None