Composite

Part:BBa_K1991008

Designed by: Chen, Pei-En   Group: iGEM16_Mingdao   (2016-10-12)

Pcons-RBS-LO-AOX1-His

The gene of Lpp-OmpA-AOX1 fusion protein (BBa_K1991005) is driven by a constitutive promoter (BBa_J23101) and RBS (BBa_B0034).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 2498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 497
    Illegal XhoI site found at 2507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2189


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Categories
Parameters
None