Coding

Part:BBa_K1991002

Designed by: Chen, Pei-En   Group: iGEM16_Mingdao   (2016-10-12)


Pcons-RBS-AOX1 (Alcohol Oxidase)

Alcohol oxidase (AOX) is an oxido-reductase that catalyzes the oxidation of alcohol and produce aldehyde and hydrogen peroxide. The substrates of AOX are primary alcohols including methanol and some short-chain alcohols such as ethanol. AOX is present in the methylotrophic yeast like Pichia pastoris, which has two genes (i.e., AOX1 and AOX2) under a strong methanol inducible promoter. The yeast uses AOX genes to metabolize methanol for providing a carbon and energy source. The AOX enzymes were used in biosensors (Biosens Bioelectron. 2005).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1748


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Categories
Parameters
None