Reporter

Part:BBa_K199098

Designed by: Clifton Davis   Group: iGEM09_MoWestern_Davidson   (2009-10-23)


CUACC with RFP Reporter

Suppressor tRNA expressed by the promoter, plac, upstream of RFP with a 5-bp insertion, CUACC. The tRNA is designed to suppress the insertion, allowing the appropriate translation of functional RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 246
    Illegal AgeI site found at 1165
    Illegal AgeI site found at 1277
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None