Device

Part:BBa_K199096

Designed by: Romina Clemente   Group: iGEM09_MoWestern_Davidson   (2009-10-19)


pTet-CCCUC_tRNA-pLac-RBS-CCCUC_RFP

This is a suppressor tRNA (anticodon CGGUC) plus a reporter gene (RFP) with a 5 bp insertion. The tRNA suppresses the insertion so that the RFP sequence can be properly read through.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 999
    Illegal AgeI site found at 1111
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apTet-CCCUC_tRNA-pLac-RBS-CCCUC_RFP