Reporter

Part:BBa_K199077

Designed by: Clifton Davis   Group: iGEM09_MoWestern_Davidson   (2009-09-20)

RBS RFP with CUAGC Addition

This RBS RFP reporter gene is a modification of BBa_E1010 with BBa_B0034; it has a 5-bp addition, CUAGC, after the start codon and before the rest of the gene. This is used in conjunction with the CUAGC tRNA(Part K199016). These CUAGC tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.

There is also a 25 bp addition after the double stop of the gene and before the Bio Brick Suffix. This addition is presumed to not effect the translation of the protein.



Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 578
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None