Device

Part:BBa_K199057

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-08-05)

CCAUC tRNA (10-bp Anticodon) with RFP Reporter

Suppressor tRNA expressed by the weak promoter, pBad, upstream of pLac+RFP with a 5-bp insertion, CCAUC. The tRNA is designed to suppress the insertion, allowing the appropriate translation of functional RFP. Induction requires Arabinose and IPTG.

See Part:BBa_K199057:Experience for characterization of this device.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 176
    Illegal AgeI site found at 1076
    Illegal AgeI site found at 1188
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 189


[edit]
Categories
Parameters
n/aCCAUC tRNA (10-bp Anticodon) with RFP Reporter