Composite

Part:BBa_K199056

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-08-05)


CCAUC tRNA (9-bp Anticodon) with RFP Reporter

Suppressor tRNA expressed by the medium-weak promoter, pBad, upstream of RFP with a 5-bp insertion, CCAUC. The tRNA is designed to suppress the insertion, allowing the appropriate translation of functional RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 176
    Illegal AgeI site found at 1075
    Illegal AgeI site found at 1187
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aCCAUC tRNA (9-bp Anticodon) with RFP Reporter