Composite

Part:BBa_K199027

Designed by: Kin Lau   Group: iGEM09_MoWestern_Davidson   (2009-07-08)


RFPrev-RBSrev-pLux

This is inverted RBS-RFP ligated upstream of pLux in forward orientation. This is intended to measure the backward activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 707


[edit]
Categories
Parameters
n/aRFPrev-RBSrev-pLux