Composite

Part:BBa_K199025

Designed by: Kin Lau   Group: iGEM09_MoWestern_Davidson   (2009-07-08)


Tetrev-RBSrev-pOmpC (with intrinsic RBS)

This part is inverted Tet-RBS ligated upstream of pOmpC with an RBS intrinsic to pOmpC as found in E. coli naturally. This part is intended to measure the backward activity of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 897
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 503
    Illegal NgoMIV site found at 871
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTetrev-RBSrev-pOmpC (with intrinsic RBS)