RNA

Part:BBa_K199016

Designed by: Clifton Davis   Group: iGEM09_MoWestern_Davidson   (2009-07-02)

CUAGC tRNA Suppressor (Produces Serine)

A serine tRNA was modified to have the anticodon loop 5' CUGCUAGAA 3'so that the anticodon GCUAG could be used to decode a frameshift mutation of CUAGC in a mRNA. It is used in conjuction with the Parts K199015 and K199033. Update: these tRNA parts do not work as expected, but instead appear to read four-base codons. See [http://www.ibc7.org/article/journal_v.php?sid=287]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aCUAGC tRNA Suppressor (Produces Serine)