RNA
Part:BBa_K199014
Designed by: William Vernon Group: iGEM09_MoWestern_Davidson (2009-07-02)
AGGAC tRNA Suppressor (Produces Serine)
A serine tRNA was modified to have the anticodon loop 5' CUGUCCUAA 3'so that the anticodon GUCCU could be used to decode a frameshift mutation of AGGAC in a mRNA. Update: these tRNA parts do not work as expected, but instead appear to read four-base codons. See [http://www.ibc7.org/article/journal_v.php?sid=287]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 38
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
n/a | AGGAC tRNA Suppressor (Produces Serine) |