Reporter

Part:BBa_K199010

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-06-29)

Tet Resistance with CCAUC Addition

This Tet reporter gene is a modification of BBa_J31007; it has a 5-bp addition, CCAUC, after the start codon and before the rest of the gene. This is used in conjunction with the CCAUC tRNA (Parts K199007 and K199008). These CCAUC tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 295
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 321
    Illegal NgoMIV site found at 689
    Illegal NgoMIV site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTet Resistance with CCAUC Addition