RNA

Part:BBa_K199008

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-06-29)

CCAUC Suppressor tRNA (10-bp Anticodon and Produces Serine)

This gene encodes for a suppressor tRNA that suppresses a 5-bp codon CCAUC, which would normally cause a frame shift mutation. Parts K199009 and K199010). Update: these tRNA parts do not work as expected, but instead appear to read four-base codons. See [http://www.ibc7.org/article/journal_v.php?sid=287]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 38
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 51


[edit]
Categories
Parameters
n/aCCAUC Suppressor tRNA (10-bp Anticodon and Produces Serine)