Reporter

Part:BBa_K199006

Designed by: Shamita Punjabi   Group: iGEM09_MoWestern_Davidson   (2009-06-26)

Tet Resistance with CCACU Addition

This Tet reporter gene is a modification of BBa_J31007; it has a 5-base pair CUAGU addition after the start codon and before the rest of the gene. This is used in conjunction with the CUAGU tRNA K199002). This CUAGU tRNA suppresses the frameshift caused by this 5-base pair addition in the reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 295
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 321
    Illegal NgoMIV site found at 689
    Illegal NgoMIV site found at 849
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTet Resistance with CCACU Addition