Reporter

Part:BBa_K199005

Designed by: Shamita Punjabi   Group: iGEM09_MoWestern_Davidson   (2009-06-26)

RFP with CCACU Addition

This RFP reporter gene is a modification of BBa_E1010; it has a 5-base pair CCACU addition after the start codon and before the rest of the gene. This is used in conjunction with the CCACU tRNA (BioBrick part K199002). This CCACU tRNA suppresses the frameshift caused by this 5-base pair addition in the reporter.

There is also a 25 bp addition after the double stop of the gene and before the Bio Brick Suffix. This addition is presumed to not effect the translation of the protein.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 560
    Illegal AgeI site found at 672
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRFP with CCACU Addition