Reporter
Part:BBa_K199004
Designed by: Romina Clemente Group: iGEM09_MoWestern_Davidson (2009-06-26)
Tet Resistance with CUAGU Addition
This Tet reporter gene is a modification of BBa_J31007; This Tet resistance reporter gene has a 5-base pair CUAGU addition after the start codon and before the rest of the gene. This is used in conjunction with the CUAGU tRNA (BioBrick part K199001)). This CUAGU tRNA suppresses the frameshift caused by this 5-base pair addition in the reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 149
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 295
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 321
Illegal NgoMIV site found at 689
Illegal NgoMIV site found at 849 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | Tet Resistance with CUAGU Addition |