Designed by: Caroline Blassick Group: iGEM16_UIUC_Illinois (2016-10-14)
On->off promoter 2
This promoter can be inserted upstream of any gene in order to provide timed gene expression. This promoter is active during the early growth phase of e. coli, but becomes less active later on, leading to an "on -> off" expression pattern.
- Note that this promoter also includes an unidentified RBS somewhere in the sequence. This promoter+RBS combination is intended to be treated as a single regulatory unit which cannot be subdivided, which is why it has been submitted as a "basic part."
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000INCOMPATIBLE WITH RFCIllegal BsaI site found at 232